{"id":10572,"date":"2024-04-11T15:14:32","date_gmt":"2024-04-11T13:14:32","guid":{"rendered":"https:\/\/www.appetizerblog.com\/?p=10572"},"modified":"2024-07-09T08:41:40","modified_gmt":"2024-07-09T06:41:40","slug":"how-to-measure-pet-food-oxidation","status":"publish","type":"post","link":"https:\/\/www.appetizerblog.com\/en\/how-to-measure-pet-food-oxidation\/","title":{"rendered":"How to measure pet food oxidation"},"content":{"rendered":"<div class=\"intro\">Oxidation affects the organoleptic and nutritional quality of all aging pet foods. Monitoring relevant oxidation compounds and residual antioxidant levels in a product, in real time or accelerated studies, is the most pertinent way to properly assess oxidative stability over time.<\/div>\n<p>&nbsp;<\/p>\n<p>Just like you, food products cannot escape the effect of time. Cheese and wine improve with age; however, this is unfortunately not the case for pet food.<\/p>\n<p>Between the time a kibble comes out of the extruder and the time it lands in Kitty\u2019s plate, several months can elapse. During this journey, the inevitable oxidation attack can affect nutritional properties and turn nice poultry scent to rancid smell, which is far less pleasing.<\/p>\n<p>&nbsp;<\/p>\n<p>Pet food manufacturers thus need to be aware of and master the oxidative stability of their product to ensure it stays nutritious and appealing to Kitty, and her Human.<\/p>\n<p>What are the methods to assess pet food oxidative stability? What are the oxidation markers to track?<\/p>\n<p>&nbsp;<\/p>\n<p><em>Follow the steps to fully measure pet food oxidation over time\u2026<\/em><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2>Oxidative stability: choose the right indicators<\/h2>\n<p>&nbsp;<\/p>\n<p>Fat oxidation is a common phenomenon affecting the quality of aging pet food. Mainly caused by high temperature or contact with oxygen, this complex set of reactions creates oxidation compounds responsible for <a href=\"https:\/\/www.appetizerblog.com\/en\/protect-your-pet-food-from-oxidation-naturally\/\">rancid smells, off-flavors, and loss of nutritional value.<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>Antioxidants are commonly added to pet food to limit this phenomenon. Their level decreases over time as they are consumed by reactions with oxidation compounds.<\/p>\n<p>&nbsp;<\/p>\n<p>The levels of <strong>specific oxidation compounds<\/strong> and <strong>residual antioxidants<\/strong> in an aging pet food can relevantly be used as indicators to assess oxidative stability over time.<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-10565 aligncenter\" src=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Pet-food-oxidation-indicators_v3.jpg\" alt=\"\" width=\"610\" height=\"585\" srcset=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Pet-food-oxidation-indicators_v3.jpg 610w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Pet-food-oxidation-indicators_v3-300x288.jpg 300w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Pet-food-oxidation-indicators_v3-350x336.jpg 350w\" sizes=\"(max-width: 610px) 100vw, 610px\" \/><\/p>\n<p>&nbsp;<\/p>\n<h3>Chasing oxidation compounds<\/h3>\n<p>&nbsp;<\/p>\n<p>The fat oxidation mechanism happening in pet food can be summarized in 3 stages: <strong>initiation<\/strong>, <strong>propagation<\/strong>, and <strong>termination<\/strong>. Each reaction stage generates different oxidation compounds.<\/p>\n<p>&nbsp;<\/p>\n<p>During the <strong>initiation and propagation<\/strong> phases, fatty acids turn into <strong>free radicals and hydroperoxides<\/strong>, both of which are <strong>primary compounds of oxidation<\/strong>.<\/p>\n<p>&nbsp;<\/p>\n<p>During the <strong>termination<\/strong> phase, hydroperoxides decompose into secondary products of oxidation. They include <strong>volatile compounds like aldehydes<\/strong>, ketones, and short chain acids, which are responsible for unpleasant odors, as well as <strong>non-volatile compounds<\/strong> that mainly impact taste.<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-10562 aligncenter\" src=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Oxidation-compounds-evolution-over-time_v3.jpg\" alt=\"\" width=\"610\" height=\"538\" srcset=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Oxidation-compounds-evolution-over-time_v3.jpg 610w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Oxidation-compounds-evolution-over-time_v3-300x265.jpg 300w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Oxidation-compounds-evolution-over-time_v3-350x309.jpg 350w\" sizes=\"(max-width: 610px) 100vw, 610px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>Many compounds generated during oxidation are currently used as indicators of oxidation state. Of these, 3 categories, both primary and secondary, are best suited to complex matrices such as pet foods:<\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong>The hydroperoxides<\/strong> measured with the <strong>Peroxide Value,<\/strong> expressed as milliequivalents oxygen per kg\u00a0of fat (meq\/kg fat).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong>Volatile aldehydes <\/strong>like <strong>hexanal, pentanal, propanal and 2,4-decadienal<\/strong>, measured with Gas chromatography and expressed in ppm.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong>Non-volatile<\/strong> <strong>aldehydes<\/strong>, measured with the para-<strong>Anisidine Value <\/strong>(no unit).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-10730 aligncenter\" src=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/07\/SYM_PF_blog_Article_40_Main-oxidation-compounds-used-as-indicatorse_v1.jpg\" alt=\"\" width=\"610\" height=\"431\" srcset=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/07\/SYM_PF_blog_Article_40_Main-oxidation-compounds-used-as-indicatorse_v1.jpg 610w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/07\/SYM_PF_blog_Article_40_Main-oxidation-compounds-used-as-indicatorse_v1-300x212.jpg 300w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/07\/SYM_PF_blog_Article_40_Main-oxidation-compounds-used-as-indicatorse_v1-350x247.jpg 350w\" sizes=\"(max-width: 610px) 100vw, 610px\" \/><\/p>\n<p>&nbsp;<\/p>\n<blockquote><p>Each indicator provides different and complementary information. To get a complete picture of a product&#8217;s oxidation state, it is best to track several oxidation compounds during a kinetic study.<\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h3>Measuring residual antioxidants level<\/h3>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.appetizerblog.com\/en\/protect-your-pet-food-from-oxidation-naturally\/\">Antioxidants<\/a> protect food by delaying oxidative reactions. According to their nature they act differently. They can trap metals and oxygen in the initiation phase, or catch free radicals during the propagation step, thus limiting the generation of unwanted molecules.<\/p>\n<p>&nbsp;<\/p>\n<p>When several antioxidants are used, they <strong>are consumed at various rates<\/strong>, until there are none left, or the food is consumed. Antioxidant <strong>residual level assessed against initial level<\/strong> is a good indicator of the oxidation status of the pet food.<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-10550 aligncenter\" src=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Residual-antioxidants-evolution-over-time_v3.jpg\" alt=\"\" width=\"610\" height=\"667\" srcset=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Residual-antioxidants-evolution-over-time_v3.jpg 610w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Residual-antioxidants-evolution-over-time_v3-274x300.jpg 274w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_Residual-antioxidants-evolution-over-time_v3-350x383.jpg 350w\" sizes=\"(max-width: 610px) 100vw, 610px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<blockquote><p>All the antioxidants used in a product should be monitored, as they act at different stages of the oxidation reaction.<\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2><strong>Oxidative stability study: choose the method<\/strong><\/h2>\n<p><strong>\u00a0<\/strong><\/p>\n<p>Once relevant oxidation indicators have been selected, it is possible to set a protocol to follow the oxidation evolution. Two main methods exist to assess the oxidative stability of a food product over time. The major difference is based on the length of the study.<\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong>Real-time study<\/strong>: the product is stored in <strong>ambient condition<\/strong>, for <strong>as long as its expected shelf life<\/strong>. Its oxidative status is tested throughout the storage duration, with at least 6 time points.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong>Accelerated study<\/strong>: the product is stored in an incubator, at a <strong>higher temperature<\/strong> (40\u00b0C to 60\u00b0C), and sometimes with a higher relative humidity. This method speeds up reactions and therefore <strong>reduces the duration of the study by 4 to 5 times<\/strong><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-10553 aligncenter\" src=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_2-types-of-methods_v2.jpg\" alt=\"\" width=\"610\" height=\"538\" srcset=\"https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_2-types-of-methods_v2.jpg 610w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_2-types-of-methods_v2-300x265.jpg 300w, https:\/\/www.appetizerblog.com\/wp-content\/uploads\/2024\/04\/SYM_PF_blog_Article_40_2-types-of-methods_v2-350x309.jpg 350w\" sizes=\"(max-width: 610px) 100vw, 610px\" \/><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>The choice of the protocol is often made according to <strong>time<\/strong> available, <strong>cost<\/strong> constraints and product <strong>strategic<\/strong> importance.<\/p>\n<p>&nbsp;<\/p>\n<p>The <strong>real time study<\/strong> is the most accurate, but also the most time-consuming, thus more expensive. If a choice needs to be made, it could be reserved for the launch of a <strong>new product or a major change<\/strong> in formula.<\/p>\n<p>&nbsp;<\/p>\n<p>The <strong>accelerated study<\/strong> is a good option which <strong>shortens the testing<\/strong> <strong>time<\/strong> from several months to several weeks, while still being reliable, especially to compare a current antioxidant to a new one.<\/p>\n<p>&nbsp;<\/p>\n<blockquote><p><strong>It is a best practice to simultaneously run both a real-time and an accelerated study, so that if a problem occurs in the accelerated study, the real-time study can be stopped earlier. <\/strong><\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<p>Whether for a real time or an accelerated study, the <strong>testing conditions<\/strong> such as<strong> the storage duration, the temperature<\/strong>, <strong>the<\/strong> <strong>indicators, and the frequency of analysis<\/strong> must be carefully chosen to be the most representative of the product\u2019s future life.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Discover the steps to conduct reliable pet food oxidative stability studies<\/p>\n","protected":false},"author":2,"featured_media":10576,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[49],"tags":[159,791,792,161,790],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v15.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>How to measure pet food oxidation - Appetizer - Symrise Pet Food<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.appetizerblog.com\/en\/how-to-measure-pet-food-oxidation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How to measure pet food oxidation - Appetizer - Symrise Pet Food\" 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